Composite

Part:BBa_K1615013:Design

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-08-27)


MorA fused to CBDcenA+Linker in RFC25 driven by LacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2493
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal NgoMIV site found at 1648
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.


Source

Morphine-6-dehydrogenase from Pseudomonas putida. CBDcenA from Cellulomonas fimi. Lac expression cassette from iGEM10_Washington.

References